Electroporation induced transformation ofBacteroides ruminicolaandBacteroides uniformisby plasmid DNA
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چکیده
منابع مشابه
High-voltage electroporation of bacteria: genetic transformation of Campylobacter jejuni with plasmid DNA.
Electroporation permits the uptake of DNA by mammalian cells and plant protoplasts because it induces transient permeability of the cell membrane. We investigated the utility of high-voltage electroporation as a method for genetic transformation of intact bacterial cells by using the enteric pathogen Campylobacter jejuni as a model system. This report demonstrates that the application of high-v...
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Loree Heller and M. Lee Lucas Center for Molecular Delivery, University of South Florida, Tampa, FL 33612, Department of Medical Microbiology, University of South Florida, Tampa, FL 33612 __________________________________________________________________________________ * Correspondence : Loree Heller, University of South Florida, Center for Molecular Delivery, MDC16, 12901 Bruce B. Downs Blvd....
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A plasmid transformation system was developed for Bacillus polymyxa ATCC 12321 and derivatives of this strain. The method utilizes a penicillin-treated-cell technique to facilitate uptake of the plasmid DNA. Low-frequency transformation (10(-6) per recipient cell) of plasmids pC194, pBD64, and pBC16 was accomplished with this method. Selection for the transformants was accomplished on both hype...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1989
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1989.tb03560.x